P-115: Excitatory and Inhibitory Effectsof Nitric Oxide on Development The Cardiomyocyte of Rat Embryos

Background: According to increased consumption of supplements especially during pregnancy and the teratogenic impact some they on evolution the vital systems of embryos. The purpose of this study to Improve the health of the fetus and prevention of cardiovascular abnormalities as the most common cause of infant mortality. We examined the effects of L-Arginine and L-NAME administration on fetal rat heart. Materials and Methods: Forty Wister rats, weighing 200- 250 g with a mean age of 8 weeks, were divided into 5 groups after making sure that the experimental rats were pregnant. The first group received 2 mg/kg normal saline and the others received respectively 200 mg/kg L-Arginine, 20 mg/kg L-NAME and a mixture of the same doses of L-Arginine and L-NAME on 8, 9, 10 and 11th gestational days via intraperitoneal. The control group did not receive any injection. The fetal Hearts were removed on 18th gestational days, after tissue preparation and staining method (E and H) histological changes were studied by Light microscopy Olympus Cx31 and software Image tools III. Results: In L-NAME group despite the significant decrease (p <0.05) in the diameter of cells and their nuclei, irregularities in heart muscle structure and intercalated disc were observed. L-Arginine group was also associated with severe Myogenesis and Angiogenesis. Conclusion: Results of this study showed that Nitric oxide (NO) in the amounts of higher or lower than normal is associated with congenital heart diseases. Therefore, drugs and supplements should be used with caution especially during pregnancy.